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Integrated visualization of ex-vivo and in-vivo data derivatives.
The CloudCompare visualization tool is supporting both point clouds and meshes representation

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Model annotation
Bundles annotation
Cortex annotation
Bundle Segmentation
Bundle Warping

Identification and segmentation of the white matter pathways of interest on the specimen's point cloud along the dissection steps

Segmentation of the gray matter on the specimen's point cloud according to gyral/sulcal macroanatomical landmarks

Manual segmentation of the bundle of interest from the whole brain tractography reconstructed from in-vivo diffusion MR images.

Registration of the bundle tractography from the individual MRI space to the reference space of the specimen.

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3D models alignment
Affine registration
Diffeomorphic registration
Rigid registration

Computation of linear transformation between photogrammetric and MRI models of the specimen

Computation of non linear transformation between the T1W of the specimen and individual MRI

In-vivo images alignment. Registration of the tractography from DWI space to the T1W space of the same individual.

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3D models reconstruction
Photo orientation
Point cloud mesh
Epoch alignment
Brain reorientation
T1W MRI scan
Brain extraction
Surface extraction
Diffusivity model
Fiber tracking

Reconstruction of reference system for the photogrammetric acquisition and annotation of metric system

Reconstruction of the 3D model and related texture of the specimen

Rigid  transformation for the alignment of two subsequent epochs

ACPC alignment of the T1 weighted structural brain image both for individual and specimen

Acquisition of a T1 weighted structural image of the ex-vivo brain before the session of dissection

Skull removal to obtain brain volume only

Computation of the cortical mesh from the T1 weighted MR image of the ex-vivo brain

Computation of constrained spherical deconvolution model

Computation of the whole brain tractogram with CSD model

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Data acquisition
Specimen dissection
Specimen setup
Photogrammetric acquisition
T1W MRI scan
DWI MRI scan

Iterative sessions of dissection of the cortical and subcortical tissues

Positioning of the specimen on the referenced table at the current epoch

Photogrammetric acquisition of the current epoch of dissected specimen

Acquisition of T1 weighted structural brain image

Acquisition of a sequence of Diffusion Weighted Images

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Starting point
Specimen
Healthy Individual

Klingler preparation of a single hemisphere after autopsy session (Sarubbo S et al., 2015)

Healthy volunteer individual for an MRI session imaging acquisition

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Workflow

DATA AND MODEL ACQUISITION PROCESS